**This workshop has ended. View the recording here.** Workshop 3: Experimental modalities for whole-brain connectivity mapping MARCH 5, 2021 Goal: What are state of the art, opportunities and challenges relevant to each of the technologies and approaches you represent?Draft Agenda: [Click here to download PDF version] 11:00 am ET Welcome and Series Overview John Ngai and Harriet Kung 11:10 am ET Welcome and Introduction Workshop co-leads: Galya Orr and Mark Ellisman 11:30 am ET Workshop Logistics 11:35 am ET Speaker Session 1 – Multi-scale imaging of the connectomeEach speaker will address four questions:What resolution is required to attain meaningful wiring models?Which imaging technologies could be driven to scale and most easily disseminated?Compare and contrast single large-scale efforts to democratize data collection.How do you encode dynamics in static brain maps (adding the time domain)? 11:35 am ET Multi-scale Imaging of the Connectome Jeff Lichtman 11:45 am ET Raw speed Winfried Denk 11:55 am ET Multi-scale imaging of connectomes using TEM Davi Bock 12:05 pm ET Multi-scale Imaging of Connectomes with Photons and Electrons Wei-Chung Allen Lee 12:15 pm ET Synchrotron Hard X-ray Microscopy for the ConnectomeSi Chen 12:25 pm ET tomoSEM – tomography in tSEM John Mendenhall 12:35 pm ET Q&A session 1:05 pm ET Break 1:15 pm ET Speaker Session 2 – Projectome to Connectome ImagingEach speaker will address four questions:What resolution is required to attain meaningful wiring models?Which imaging technologies could be driven to scale and most easily disseminated?Compare and contrast single large-scale efforts to democratize data collection.How do you encode dynamics in static brain maps (adding the time domain)? Synapto-Projectomes: Toward connectome relationships in target fields 1:15 pm ET Axonal connectomics: projection maps in mice, NPHs, and humansClay Reid 1:25 pm ET Mapping Projections of Melanopsin Retinal Ganglion Cells to Brain Keun-Young Kim 1:35 pm ET Connectome at the ‘operational’ resolution and in the context of protein dynamicsTianyi Mao Strategies to bridge spatial and temporal gaps 1:45 pm ET Novel Mouse Genetic Tools for Scalable Analyses of Brain Cell Morphology X. William Yang 1:55 pm ET Towards Whole-Cortical Cellular Resolution Recording of NeuroactivityAlipasha Vaziri 2:05 pm ET Next-Generation Connectomics (NGC) via optical in-situ multiplexing Adam Marblestone 2:15 pm ET Q&A session 2:45 pm ET Break 2:55 pm ET Discussion PanelPanel Discussion topic: The tyranny of scale: Challenges presented by spatial and temporal scalesPanel chairs: Moritz Helmstaedter, Hongkui ZengDiscussants: Moritz Helmstaedter, Hongkui Zeng, Anton Maximov, Kenneth Hayworth, Lisa Miller, Mark Schnitzer 3:55 pm ET Closing remarks Workshop co-leads: Mark Ellisman and Galya Orr 4:00 pm ET Workshop ends