Workshop 3: Experimental modalities for whole-brain connectivity mapping

MARCH 5, 2021​

Goal: What are state of the art, opportunities and challenges relevant to each of the technologies and approaches you represent?

Draft Agenda: [Click here to download PDF version]

11:00 am ET

Welcome and Series Overview
John Ngai and Harriet Kung

11:10 am ET

Welcome and Introduction
Workshop co-leads: Galya Orr and Mark Ellisman

11:30 am ET

Workshop Logistics

11:35 am ET

Speaker Session 1 – Multi-scale imaging of the connectome

Each speaker will address four questions:

  1. What resolution is required to attain meaningful wiring models?
  2. Which imaging technologies could be driven to scale and most easily disseminated?
  3. Compare and contrast single large-scale efforts to democratize data collection.
  4. How do you encode dynamics in static brain maps (adding the time domain)?

11:35 am ET

Multi-scale Imaging of the Connectome
Jeff Lichtman

11:45 am ET

Winfried Denk

11:55 am ET

Multi-scale imaging of connectomes using TEM
Davi Bock

12:05 pm ET

Multi-scale Imaging of Connectomes With Photons and Electrons
Wei-Chung Allen Lee

12:15 pm ET

Synchrotron Hard X-ray Microscopy for the Connectome
Si Chen

12:25 pm ET

tomoSEM – tomography in tSEM
John Mendenhall

12:35 pm ET

Q&A session

1:05 pm ET

Break

1:15 pm ET

Speaker Session 2 – Projectome to Connectome Imaging

Each speaker will address four questions:

  1. What resolution is required to attain meaningful wiring models?
  2. Which imaging technologies could be driven to scale and most easily disseminated?
  3. Compare and contrast single large-scale efforts to democratize data collection.
  4. How do you encode dynamics in static brain maps (adding the time domain)?

Synapto-Projectomes: Toward connectome relationships in target fields

1:15 pm ET

Axonal connectomics: projection maps in mice, NPHs, and humans
Clay Reid

1:25 pm ET

Mapping Projections of Melanopsin Retinal Ganglion Cells to Brain
Keun-Young Kim

1:35 pm ET

Connectome at the ‘operational’ resolution and in the context of protein dynamics
Tianyi Mao

Strategies to bridge spatial and temporal gaps

1:45 pm ET

Novel Mouse Genetic Tools for Scalable Analyses of Brain Cell Morphology
X. William Yang

1:55 pm ET

Towards Whole-Cortical Cellular Resolution Recording of Neuroactivity
Alipasha Vaziri

2:05 pm ET

Next-Generation Connectomics (NGC) via optical in-situ multiplexing
Adam Marblestone

2:15 pm ET

Q&A session

2:45 pm ET

Break

2:55 pm ET

Discussion Panel

Panel Discussion topic: The tyranny of scale: Challenges presented by spatial and temporal scales

Panel chairs: Moritz Helmstaedter, Hongkui Zeng

Discussants: Moritz Helmstaedter, Hongkui Zeng, Anton Maximov, Kenneth Hayworth, Lisa Miller, Mark Schnitzer

3:55 pm ET

Closing remarks
Workshop co-leads: Mark Ellisman and Galya Orr

4:00 pm ET

Workshop ends